9BMC
Post-2 motor domain from full-length human dynein-1 bound to microtubules in 5mM ADP condition
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.5 |
| Software | cryoSPARC,SerialEM,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
