9BLI

Crystal structure of Actin capping protein in complex with a fragment of Legionella pneumophila RavB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-05
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	66.502, 55.322, 77.900
Unit cell angles	90.00, 107.08, 90.00

Refinement procedure

Resolution	44.410 - 2.000
R-factor	0.1822
Rwork	0.180
R-free	0.20760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.712
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.124	0.067	0.359
Rmeas	0.130	0.070	0.499
Rpim	0.039	0.021	0.345
Total number of observations	330215
Number of reflections	35564	1924	1036
<I/σ(I)>	6.8
Completeness [%]	96.7	100	56.6
Redundancy	9.3	11.5	1.3
CC(1/2)	0.994	0.999	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	10 mM Tris pH 8.0, 0.2 M ammonium formate, 0.15 M NaCl, 21% w/v PEG 3350, 30% ethylene glycol