9BL4
KIR3DL1*086 in complex with HLA-B*57:03 presenting the AW10 peptide
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953659 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.936, 60.537, 64.900 |
| Unit cell angles | 95.37, 96.50, 106.02 |
Refinement procedure
| Resolution | 44.630 - 1.750 |
| R-factor | 0.1925 |
| Rwork | 0.191 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.983 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.630 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.055 | 0.297 |
| Number of reflections | 63547 | 3559 |
| <I/σ(I)> | 8.9 | 1.6 |
| Completeness [%] | 93.1 | |
| Redundancy | 1.8 | |
| CC(1/2) | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 16% PEG 3350, 2% tacsimate pH 5.0, 0.1 M tri-sodium citrate pH 5.6 |
