9BKX
Mycobacterium tuberculosis encapsulin in complex with DyP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 313.451, 313.451, 313.451 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.560 - 3.150 |
| R-factor | 0.2026 |
| Rwork | 0.201 |
| R-free | 0.23270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.527 |
| Data reduction software | MOSFLM |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.560 | 3.200 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.204 | 0.887 |
| Rmeas | 0.257 | 1.125 |
| Rpim | 0.155 | 0.683 |
| Number of reflections | 174789 | 8661 |
| <I/σ(I)> | 7.4 | 1.6 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 4.5 | 4.5 |
| CC(1/2) | 0.957 | 0.507 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Trimethlyamine N-oxide (TMN), 0.1 M Tris-HCl pH 8.5, and 20% polyethylene glycol MME 2000 |
