9BKH
Crystal structure of RidA family protein PA5083 from Pseudomonas aeruginosa with Acetaldehyde bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-28 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 2 |
Unit cell lengths | 75.332, 75.332, 139.794 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.670 - 1.670 |
R-factor | 0.1898 |
Rwork | 0.187 |
R-free | 0.21310 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.060 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.700 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.055 | 0.545 |
Number of reflections | 17900 | 783 |
<I/σ(I)> | 35.6 | |
Completeness [%] | 99.3 | |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M BIS-TRIS pH 5.5, 2.0 M Ammonium sulfate |