9BKB

Crystal structure of Rid family protein ACIAD3089 from Acinetobacter baylyi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-09
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	87.410, 87.410, 183.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.280 - 2.100
R-factor	0.1997
Rwork	0.199
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.939
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.280	2.400
High resolution limit [Å]	2.100	2.100
Rmerge	0.074	0.558
Number of reflections	68037	2648
<I/σ(I)>	21.9
Completeness [%]	94.2
Redundancy	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG 3350, 0.2M sodium sulfate, Reductive methylation