9BKA
Crystal structure of Rid family protein PSPTO3006
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-23 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 3 |
Unit cell lengths | 71.307, 71.307, 71.307 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.170 - 1.860 |
R-factor | 0.1743 |
Rwork | 0.172 |
R-free | 0.19840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.841 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.890 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.069 | 0.316 |
Number of reflections | 10209 | 384 |
<I/σ(I)> | 31.79 | 2.23 |
Completeness [%] | 98.1 | |
Redundancy | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.8 M Potassium sodium tartrate tetrahydrate,0.1 M Tris pH 8.5,0.5% w/v Polyethylene glycol monomethyl ether 5,000 |