9BK4

Crystal structure of RidA family protein PA5083 from Pseudomonas aeruginosa with pyruvic acid bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-25
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	H 3 2
Unit cell lengths	75.404, 75.404, 279.606
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.720 - 2.080
R-factor	0.1876
Rwork	0.184
R-free	0.21850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.894
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.080	2.080
Rmerge	0.045	0.143
Number of reflections	18793	911
<I/σ(I)>	43.57
Completeness [%]	99.7
Redundancy	6.1
CC(1/2)		0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M BIS-TRIS pH 5.5, 2.0 M Ammonium sulfate