9BJY
Crystal structure of RidA family protein PA5083 from Pseudomonas aeruginosa with TMAO bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-13 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 2 |
Unit cell lengths | 75.280, 75.280, 279.505 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.670 - 1.580 |
R-factor | 0.1827 |
Rwork | 0.181 |
R-free | 0.21240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.092 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.610 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.049 | 0.453 |
Number of reflections | 40206 | 2915 |
<I/σ(I)> | 19.5 | 2.13 |
Completeness [%] | 95.0 | 99.1 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M BIS-TRIS pH 5.5, 2.0 M Ammonium sulfate |