9BJF

Tungstate binding protein (Tungbindin) from Eubacterium limosum in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-23
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	75.441, 75.441, 248.697
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.980 - 2.890
R-factor	0.288
Rwork	0.284
R-free	0.32270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.365
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.980	2.950
High resolution limit [Å]	2.890	2.900
Rmerge	0.177	0.788
Rmeas	0.190	0.856
Rpim	0.067
Number of reflections	29099	484
<I/σ(I)>	9.8	2
Completeness [%]	99.1
Redundancy	7.7
CC(1/2)		0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.1 M Sodium malonate pH 7.0, 0.1 M HEPES pH 7.0, 0.5% v/v Jeffamine ED-2001 pH 7.0