9BIZ
Crystal structure of the periplasmic domain of IgaA from Klebsiella pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2021-11-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97911 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.514, 146.443, 52.981 |
Unit cell angles | 90.00, 120.53, 90.00 |
Refinement procedure
Resolution | 39.000 - 2.500 |
R-factor | 0.1967 |
Rwork | 0.195 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.054 |
Data reduction software | HKL-3000 |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rpim | 0.103 | |
Number of reflections | 17504 | 649 |
<I/σ(I)> | 1.05 | |
Completeness [%] | 94.9 | |
Redundancy | 4.1 | |
CC(1/2) | 0.503 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.0 M lithium sulfate, 0.1 M Bis-Tris, pH 7.0 |