9BIV
Crystal Structure of Ubc13 with a New Active Site Loop Conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-15 |
Detector | DECTRIS PILATUS3 R 200K-A |
Wavelength(s) | 1.54178 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 86.361, 42.942, 93.790 |
Unit cell angles | 90.00, 108.37, 90.00 |
Refinement procedure
Resolution | 42.684 - 1.680 |
R-factor | 0.1454 |
Rwork | 0.144 |
R-free | 0.17660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.803 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.16_3546: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmeas | 0.286 | |
Number of reflections | 37365 | 1843 |
<I/σ(I)> | 5.37 | |
Completeness [%] | 99.9 | |
Redundancy | 7.6 | |
CC(1/2) | 0.940 | 0.940 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7.5 | 298 | Ubc13-Mms2 heterodimer was concentrated to 12 mg/mL and stored in 50 mM Tris pH 7.5, 150 mM NaCl, 1mM TCEP. Complex was left to slowly equilibrate from 277K to 298 overnight |