9BIG

Stat6 bound to degrader AK-1690

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.127
Spacegroup name	P 31
Unit cell lengths	103.505, 103.505, 53.059
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.880 - 3.304
R-factor	0.2092
Rwork	0.205
R-free	0.28870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.900
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (8-JUN-2022))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.280
High resolution limit [Å]	3.220	8.730	3.220
Rmerge	0.076	0.037	1.028
Rmeas	0.080	0.039	1.087
Rpim	0.024	0.012	0.353
Total number of observations	106933
Number of reflections	10231	512	497
<I/σ(I)>	7.5
Completeness [%]	99.6	99.6	100
Redundancy	10.5	11	9.2
CC(1/2)	0.995	0.999	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.1 M succinic acid pH 7.0, 15% (w/v) PEG 3350, 6 mM strontium chloride hexahydrate, and 10% mixed additive (0.33% w/v 1,5-Naphthalenedisulfonic acid disodium salt, 0.33% w/v Naphthalene-1,3,6-trisulfonic acid trisodium salt hydrate, 0.33% w/v PIPES, and 0.02 M HEPES sodium pH 6.8)