9BGP
X-ray structure of the aminotransferase from Vibrio vulnificus responsible for the biosynthesis of 2,3-diacetamido-4-amino-2,3,4-trideoxy-arabinose in the presence of its internal aldimine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-22 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.110, 53.116, 70.322 |
| Unit cell angles | 82.59, 82.56, 74.40 |
Refinement procedure
| Resolution | 33.450 - 1.250 |
| R-factor | 0.16848 |
| Rwork | 0.167 |
| R-free | 0.18813 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.520 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 199391 | 39859 |
| <I/σ(I)> | 10.7 | 2.8 |
| Completeness [%] | 98.6 | 95.6 |
| Redundancy | 4.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 8-12% (w/v) poly(ethylene glycol) 8000, 200 mM LiCl, and 100 mM Homo-PIPES (pH 5.0) |
