Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.19499 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.996, 104.704, 66.256 |
| Unit cell angles | 90.00, 90.86, 90.00 |
Refinement procedure
| Resolution | 55.970 - 1.510 |
| R-factor | 0.1893 |
| Rwork | 0.187 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.288 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.970 | 1.550 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.087 | 0.892 |
| Rpim | 0.055 | 0.575 |
| Number of reflections | 100049 | 7353 |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 97.8 | 97.2 |
| Redundancy | 3.4 | |
| CC(1/2) | 0.988 | 0.591 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 28% PEG 3350, 0.1M Bis Tris, pH 5.5, 150 mM NaCl |
