Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-05 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | .953 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.210, 58.080, 75.950 |
| Unit cell angles | 90.00, 97.95, 90.00 |
Refinement procedure
| Resolution | 35.070 - 1.200 |
| R-factor | 0.1223 |
| Rwork | 0.121 |
| R-free | 0.14750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.171 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.070 | 1.243 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.044 | 0.199 |
| Rmeas | 0.051 | 0.237 |
| Rpim | 0.026 | 0.126 |
| Number of reflections | 99659 | 9775 |
| <I/σ(I)> | 15.35 | 4.98 |
| Completeness [%] | 99.6 | 98.16 |
| Redundancy | 3.7 | 3.2 |
| CC(1/2) | 0.998 | 0.947 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 25% PEG 3350, 0.1M Bis Tris, pH 5.5 |
