9BF0

MID domain of human Argo2 bound to UTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	Excillum MetalJet D2+ 70 kV
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-19
Detector	Bruker PHOTON III
Wavelength(s)	1.3418
Spacegroup name	P 1
Unit cell lengths	40.106, 46.511, 65.240
Unit cell angles	87.78, 74.53, 85.41

Refinement procedure

Resolution	20.530 - 1.780
R-factor	0.1905
Rwork	0.188
R-free	0.23360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.737
Data reduction software	PROTEUM PLUS
Data scaling software	SAINT
Phasing software	MOLREP
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.530	1.844
High resolution limit [Å]	1.780	1.780
Number of reflections	86315	8459
<I/σ(I)>	5.72
Completeness [%]	98.9
Redundancy	3.9
CC(1/2)	0.994	0.273

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	drop was 1:1 15 mg/ml protein with RNA: 1.4 M Sodium citrate tribasic dihydrate, 0.1 M HEPES pH 7.5