9BEY
Structure of a lambda-carrageenan active GH2 A in complex with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-14 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.450, 70.274, 89.186 |
| Unit cell angles | 90.00, 113.48, 90.00 |
Refinement procedure
| Resolution | 29.676 - 2.386 |
| R-factor | 0.1912 |
| Rwork | 0.189 |
| R-free | 0.23110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.540 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.386 | 2.386 |
| Rmerge | 0.121 | 0.318 |
| Rpim | 0.058 | 0.231 |
| Number of reflections | 38258 | 1529 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 97.9 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.987 | 0.821 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 15% PEG 8000, 0.1 M sodium cacodylate, 0.2 M calcium acetate, 0.2 M LiCl, 5% glycerol |
