9BEO

Tungstate binding protein (Tungbindin) from Eubacterium limosum with 7.5 tungstates bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-19
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	74.752, 74.752, 156.577
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.140 - 1.780
R-factor	0.1804
Rwork	0.180
R-free	0.19470
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.995
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	AutoSol
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.140	1.810
High resolution limit [Å]	1.780	1.780
Rmerge	0.097	0.446
Rmeas	0.100	0.497
Rpim	0.022	0.210
Number of reflections	77439	1071
<I/σ(I)>	52.7	3.76
Completeness [%]	96.5	70.7
Redundancy	18	5
CC(1/2)		0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Sodium chloride, 0.1 M BIS-TRIS pH 6.5, 25% w/v Polyethylene glycol 3,350