9BEM

Tungstate binding protein (Tungbindin) from Eubacterium limosum with seven Tungstates bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-12
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	74.751, 74.751, 156.584
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.810 - 2.260
R-factor	0.1829
Rwork	0.178
R-free	0.22660
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.049
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	AutoSol
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.260	2.260
Rmerge	0.122	0.577
Rmeas	0.126	0.651
Rpim	0.029	0.291
Number of reflections	37361	482
<I/σ(I)>	31.3	2.94
Completeness [%]	95.3
Redundancy	16.3
CC(1/2)		0.824

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium sulfate, 0.1 M MES pH 6.5, 20%(w/v) PEG 8000