9BED

Tungstate binding protein (Tungbindin) from Eubacterium limosum with eight molybdates bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-19
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	74.713, 74.713, 156.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.360 - 2.020
R-factor	0.1543
Rwork	0.151
R-free	0.20480
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.216
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	AutoSol
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.360	2.050
High resolution limit [Å]	2.020	2.020
Rmerge	0.105	0.300
Rmeas		0.337
Rpim	0.023	0.149
Number of reflections	54278	916
<I/σ(I)>	47	5.93
Completeness [%]	97.7
Redundancy	18.9
CC(1/2)		0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Magnesium acetate, 0.1 M Sodium cacodylate pH 6.5, 15%(w/v) PEG 6000