9BEA
Structure of SARS-CoV-2 full-length WT spike protein with internal tag, 2RBD-up conformation (SPIKE-WT)
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 BIOCONTINUUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.85 |
| Software | cryoSPARC,UCSF Chimera,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
