9BE2

Structure of the E. coli nucleic associated protein, YejK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 43 2 2
Unit cell lengths	85.124, 85.124, 113.797
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.560 - 3.560
R-factor	0.2745
Rwork	0.272
R-free	0.29650
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.383
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.560	4.050
High resolution limit [Å]	3.560	3.560
Rpim	0.031	0.070
Number of reflections	4762	478
<I/σ(I)>	18.1	2.1
Completeness [%]	88.2
Redundancy	7.4
CC(1/2)	0.999	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	1.8 M sodium/potassium phosphate, 0.1 M HEPES pH 7.5