9BE2
Structure of the E. coli nucleic associated protein, YejK
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 85.124, 85.124, 113.797 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.560 - 3.560 |
R-factor | 0.2745 |
Rwork | 0.272 |
R-free | 0.29650 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.383 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.560 | 4.050 |
High resolution limit [Å] | 3.560 | 3.560 |
Rpim | 0.031 | 0.070 |
Number of reflections | 4762 | 478 |
<I/σ(I)> | 18.1 | 2.1 |
Completeness [%] | 88.2 | |
Redundancy | 7.4 | |
CC(1/2) | 0.999 | 0.886 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 1.8 M sodium/potassium phosphate, 0.1 M HEPES pH 7.5 |