9BE0
GC-centric NF-kappaB RelA binding DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.857, 46.850, 99.289 |
| Unit cell angles | 90.14, 90.20, 60.02 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.28876 |
| Rwork | 0.288 |
| R-free | 0.29748 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.947 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.062 | 0.400 |
| Number of reflections | 23456 | 1431 |
| <I/σ(I)> | 17 | 2.2 |
| Completeness [%] | 90.8 | 73.8 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG3350 8%, Sodium Acetate pH 3.5 100 mM, Zinc Acetate 50 mM |
