Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-22 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.310, 99.123, 59.324 |
| Unit cell angles | 90.00, 107.73, 90.00 |
Refinement procedure
| Resolution | 49.560 - 2.000 |
| R-factor | 0.1987 |
| Rwork | 0.198 |
| R-free | 0.21950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.591 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.080 | 1.900 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.079 | 1.972 |
| Rpim | 0.032 | 0.848 |
| Number of reflections | 50481 | 2539 |
| <I/σ(I)> | 13.2 | 0.8 |
| Completeness [%] | 97.2 | 74.5 |
| Redundancy | 6.9 | 5.9 |
| CC(1/2) | 0.999 | 0.454 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.02 M of each Monosaccharide II 0.1 M MOPSO/bis-tris |
