9BBM
PHF filament generated from 4E-Tau(297-407) under neutral Mg2+ condition
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOCONTINUUM (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.2 |
| Number of Particle | 651965 |
| Software | RELION |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
