9BBI
Co-crystal structure of human DDB1 bound to fragment UB028669
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-25 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.039, 125.742, 169.937 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.010 - 1.900 |
| R-factor | 0.18998 |
| Rwork | 0.188 |
| R-free | 0.22060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.395 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.010 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.125 | 0.726 |
| Rmeas | 0.135 | 0.786 |
| Rpim | 0.052 | 0.298 |
| Total number of observations | 35004 | |
| Number of reflections | 106548 | 5233 |
| <I/σ(I)> | 7.2 | 1.7 |
| Completeness [%] | 99.4 | |
| Redundancy | 6.4 | 6.7 |
| CC(1/2) | 0.995 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% (v/w) PEG 3350, 0.2 M di-NH4tart |
