9BBI

Co-crystal structure of human DDB1 bound to fragment UB028669

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-25
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	63.039, 125.742, 169.937
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.010 - 1.900
R-factor	0.18998
Rwork	0.188
R-free	0.22060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.395
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.010	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.125	0.726
Rmeas	0.135	0.786
Rpim	0.052	0.298
Total number of observations		35004
Number of reflections	106548	5233
<I/σ(I)>	7.2	1.7
Completeness [%]	99.4
Redundancy	6.4	6.7
CC(1/2)	0.995	0.755

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% (v/w) PEG 3350, 0.2 M di-NH4tart