9BBH
Co-crystal structure of human DDB1 bound to fragment UB028670
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-09-14 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.924, 125.268, 168.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.820 - 2.000 |
| R-factor | 0.18992 |
| Rwork | 0.188 |
| R-free | 0.23191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.135 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.820 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.819 |
| Rmeas | 0.117 | 0.875 |
| Rpim | 0.041 | 0.305 |
| Total number of observations | 34490 | |
| Number of reflections | 90240 | 4336 |
| <I/σ(I)> | 16.7 | 2.7 |
| Completeness [%] | 99.4 | |
| Redundancy | 8 | 8 |
| CC(1/2) | 0.999 | 0.751 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% (v/w) PEG 3350, 0.2 M di-NH4tart |
