9BBE

Co-crystal structure of human DDB1 bound to fragment UB028668

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-25
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	63.319, 126.163, 170.313
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.180 - 2.000
R-factor	0.20935
Rwork	0.208
R-free	0.23945
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.264
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.180	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.123	0.780
Rmeas	0.133	0.840
Rpim	0.049	0.306
Total number of observations		33183
Number of reflections	92662	4542
<I/σ(I)>	7	1.4
Completeness [%]	99.7
Redundancy	7	7.3
CC(1/2)	0.993	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% (v/w) PEG 3350, 0.2 M di-NH4tart