9BAM

Surface glycan-binding protein A (SGBP-A, SusD-like) from a mixed-linkage beta-glucan utilization locus in Segatella copri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	68.877, 76.849, 99.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.560 - 2.050
R-factor	0.1872
Rwork	0.185
R-free	0.23310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.848
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.560	2.123
High resolution limit [Å]	2.050	2.050
Rmerge	0.045	0.289
Number of reflections	33784	3338
<I/σ(I)>	8.41	2.24
Completeness [%]	99.3
Redundancy	2
CC(1/2)	0.998	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Tris-HCl (pH 8.5) and 26% (w/v) Polyethylene glycol (PEG) 8,000