9BAL
Surface glycan-binding protein A (SGBP-A, SusD-like) from a mixed-linkage beta-glucan utilization locus in Segatella copri in complex with cellopentaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-03 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.953720 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.960, 77.410, 100.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.030 - 1.500 |
| R-factor | 0.1502 |
| Rwork | 0.149 |
| R-free | 0.16670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.030 | 1.554 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.016 | 0.139 |
| Number of reflections | 86193 | 8470 |
| <I/σ(I)> | 18.83 | |
| Completeness [%] | 99.8 | |
| Redundancy | 2 | |
| CC(1/2) | 1.000 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris-HCl (pH 8.5) and 23% (w/v) Polyethylene glycol (PEG) 8,000 |
