9BAG
Crystal structure of Oryza sativa ketol-acid reductoisomerase in complex with Mg2+, and JK-5-114
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-23 |
| Detector | DECTRIS EIGER2 R 1M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.410, 91.863, 86.998 |
| Unit cell angles | 90.00, 101.37, 90.00 |
Refinement procedure
| Resolution | 45.930 - 1.960 |
| R-factor | 0.2135 |
| Rwork | 0.213 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.664 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.010 | 2.000 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rpim | 0.035 | 0.566 |
| Number of reflections | 473997 | 22895 |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 97.8 | 79.8 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M NH4SO4 (Salt) 0.1 M BIS-TRIS 6.5 pH (Buffer) 25 %w/v PEG 3350 (Precipitant) |
