9BA7

Crystal structure of Vibrio cholerae N150T NFeoB variant with a single GDP molecule bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97934
Spacegroup name	P 21 21 2
Unit cell lengths	202.466, 50.093, 62.249
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.940 - 2.880
R-factor	0.2167
Rwork	0.214
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.155
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.940	2.930
High resolution limit [Å]	2.880	2.880
Number of reflections	14956	4319
<I/σ(I)>	8.8
Completeness [%]	99.4
Redundancy	5.3
CC(1/2)	0.997	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	24% (w/v) PEG 3350, 0.1 M bis-Tris (pH 5.5), 30 mM magnesium chloride, 0.2 M ammonium sulfate, 25 mM Tris (pH 8), 5% (w/v) glycerol, 100 mM NaCl, 1 mM TCEP*HCl, 3 mM GDP