9B9L
RPRD1B C-terminal interacting domain bound to a pThr4 CTD peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-13 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 66.317, 90.231, 135.074 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.790 - 2.500 |
R-factor | 0.2737 |
Rwork | 0.266 |
R-free | 0.34700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.314 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
Rmerge | 0.051 | 0.494 | |
Rmeas | 0.059 | 0.562 | |
Rpim | 0.030 | 0.260 | |
Number of reflections | 13291 | 775 | 407 |
<I/σ(I)> | 5.1 | ||
Completeness [%] | 92.1 | 97 | 56.4 |
Redundancy | 3.9 | 3.8 | 3.7 |
CC(1/2) | 0.930 | 0.996 | 0.886 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Co-crystallization of CID with CTD peptide occurred in a reservoir solution of 20-32% PEG3350 and 0.1M lithium sulfate. 1:3 molar ratio of protein to peptide was used |