9B75

Crystal structure of humanized 44H10 Fab Version 17

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	36.923, 84.844, 64.778
Unit cell angles	90.00, 106.42, 90.00

Refinement procedure

Resolution	35.420 - 1.950
R-factor	0.1819
Rwork	0.180
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.666
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.980
High resolution limit [Å]	1.950	1.950
Number of reflections	27394	1054
<I/σ(I)>	8.6
Completeness [%]	97.9
Redundancy	3.4
CC(1/2)	0.993	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	20% PEG3350, 0.2 M potassium nitrate pH 6.9