9B74

Crystal structure of humanized 44H10 Fab Version 14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 31 2 1
Unit cell lengths	123.325, 123.325, 90.714
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.880 - 2.480
R-factor	0.1759
Rwork	0.174
R-free	0.20520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.873
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.520
High resolution limit [Å]	2.480	2.480
Number of reflections	28548	1351
<I/σ(I)>	6.1
Completeness [%]	99.8
Redundancy	3.9
CC(1/2)	0.977	0.506

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	15% glycerol, 25.5% PEG4000, 0.17 M sodium acetate, 0.085 M Tris pH 8.5