9B50

E. coli 70S ribosome complex (unmethylated 16S A1408 + arbekacin)

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	GIF Bioquantum
Detector	GATAN K3 BIOQUANTUM (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.7
Number of Particle	1620093
Software	RELION,PHENIX,Topaz,CTFFIND,UCSF ChimeraX
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION