9B4Z
E. coli 70S ribosome complex (N1-methylated 16S A1408 + arbekacin)
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | TFS FALCON 4i (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.2 |
| Number of Particle | 356507 |
| Software | RELION,Topaz,PHENIX,UCSF ChimeraX,CTFFIND |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
