9B4R

Crystal structure of MRAS bound to GMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.19556
Spacegroup name	P 42 21 2
Unit cell lengths	65.481, 65.481, 107.806
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.540 - 2.100
R-factor	0.2015
Rwork	0.199
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.533
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.540	42.540	2.220
High resolution limit [Å]	2.100	6.240	2.100
Rmerge	0.102	0.038	0.747
Rmeas	0.109	0.041	0.806
Number of reflections	14328	647	2214
<I/σ(I)>	15.17
Completeness [%]	99.2
Redundancy	8
CC(1/2)	0.999	0.999	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	0.002 M divalent II mix, 50%v/v(30%w/v PEG 3000, 40%v/v 1,2,4-butanetriol, and 2%w/v NDSB 256)