9B4Q

Crystal structure of RRAS2 (RAS-Related protein) bound to GMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	39.299, 62.797, 66.089
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.520 - 1.460
R-factor	0.1836
Rwork	0.182
R-free	0.21610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.316
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.520	45.520	1.540
High resolution limit [Å]	1.460	4.350	1.460
Rmerge	0.083	0.051	1.230
Rmeas	0.087	0.053	1.284
Number of reflections	29226	1214	4594
<I/σ(I)>	15.46
Completeness [%]	99.8
Redundancy	12.6
CC(1/2)	0.999	0.998	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	293	0.1 M Tris, 5%w/v PGA-LM, 30%w/v PEG MME 550