9B4P
Tetramer Formation of the BCL11A ZF0 Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 93.15 |
Detector technology | PIXEL |
Collection date | 2022-04-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979180 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.404, 58.311, 70.087 |
Unit cell angles | 90.00, 134.12, 90.00 |
Refinement procedure
Resolution | 50.310 - 2.560 |
R-factor | 0.2789 |
Rwork | 0.278 |
R-free | 0.29410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.023 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.310 | 2.649 |
High resolution limit [Å] | 2.558 | 2.558 |
Rmerge | 0.141 | 0.680 |
Rmeas | 0.169 | 0.807 |
Rpim | 0.091 | 0.431 |
Number of reflections | 31144 | 955 |
<I/σ(I)> | 5.28 | 1.19 |
Completeness [%] | 97.3 | 98.56 |
Redundancy | 3.4 | 3.4 |
CC(1/2) | 0.985 | 0.637 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.3 | 291.15 | 0.2M potassium nitrate, 0.1M Tris-HCl pH 7.3, 20% PEG3350 |