9B47
Y328F variant of P. indica phosphate transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2016-12-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.116 |
| Spacegroup name | H 3 |
| Unit cell lengths | 174.230, 174.230, 171.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 113.290 - 3.700 |
| R-factor | 0.2325 |
| Rwork | 0.225 |
| R-free | 0.25650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.550 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 113.300 | 3.830 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.140 | 2.450 |
| Rmeas | 0.153 | 2.730 |
| Rpim | 0.063 | 1.170 |
| Number of reflections | 20706 | 2060 |
| <I/σ(I)> | 8.41 | 1.12 |
| Completeness [%] | 99.3 | |
| Redundancy | 5.9 | |
| CC(1/2) | 0.998 | 0.152 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 27%(W/V) pentaerythritol propoxylate, 5% polyethylene glycol 400, 0.2M KCl, 0.1M sodium citrate |
