9B44

Crystal structure of mAb 8-24 Fab, a VRC01-like HIV-1 antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00004
Spacegroup name	P 21 21 21
Unit cell lengths	41.874, 77.699, 266.152
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.360 - 2.010
R-factor	0.2299
Rwork	0.227
R-free	0.28060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.937
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.360	2.060
High resolution limit [Å]	2.010	2.010
Rmerge	0.110	0.604
Rmeas	0.121	0.672
Rpim	0.049	0.288
Total number of observations	340358	21759
Number of reflections	58632	4214
<I/σ(I)>	10	2.4
Completeness [%]	99.1
Redundancy	5.8	5.2
CC(1/2)	0.996	0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	30% PEG3350, 0.4M KSCN