9B3S

Crystal structure of casein kinase 1 delta 1 with tethered phosphorylated tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97741
Spacegroup name	C 1 2 1
Unit cell lengths	55.452, 135.117, 90.602
Unit cell angles	90.00, 94.36, 90.00

Refinement procedure

Resolution	45.933 - 2.400
R-factor	0.1989
Rwork	0.196
R-free	0.24670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pxo
RMSD bond length	0.008
RMSD bond angle	1.333
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.933	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.175	0.873
Number of reflections	25723	2688
<I/σ(I)>	8.9	2
Completeness [%]	99.1
Redundancy	6.9
CC(1/2)	0.990	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	20% PEG3350, 0.2 M succinic acid, pH 5.5