9B3E

Crystal structure of a Slam-dependent surface lipoprotein, PmSLP, in Pasteurella multocida

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	105
Detector technology	PIXEL
Collection date	2018-02-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	58.910, 81.500, 109.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.740 - 2.000
R-factor	0.2055
Rwork	0.203
R-free	0.24490
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.892
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.13-2998)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.744	2.071
High resolution limit [Å]	2.000	2.000
Rmerge	0.109	1.366
Rmeas	0.118
Rpim	0.046	0.581
Number of reflections	67811	3359
<I/σ(I)>	11.96	1.44
Completeness [%]	98.2	93.07
Redundancy	6.4	6.5
CC(1/2)	0.998	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Na cacodylate, ammonium tartrate, PEG3350