9B2W
PIV3 HN with Fab 13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.273, 129.353, 89.454 |
| Unit cell angles | 90.00, 93.57, 90.00 |
Refinement procedure
| Resolution | 34.760 - 2.510 |
| R-factor | 0.2214 |
| Rwork | 0.219 |
| R-free | 0.26440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.692 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmeas | 0.481 | |
| Rpim | 0.897 | |
| Number of reflections | 61212 | 5863 |
| <I/σ(I)> | 17.8 | |
| Completeness [%] | 97.0 | |
| Redundancy | 1.6 | |
| CC(1/2) | 0.985 | 0.897 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | 0.1M Ammonium sulfate, 0.1M MES pH 6.5, 15% (w/v) PEG 4000 |
