9B2O
Structure of the quorum quenching lactonase GcL bound to the hydrolysis product of N-octanoyl-L-homoserine lactone
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03321 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.360, 109.810, 97.370 |
| Unit cell angles | 90.00, 116.35, 90.00 |
Refinement procedure
| Resolution | 74.130 - 1.850 |
| R-factor | 0.18814 |
| Rwork | 0.187 |
| R-free | 0.21475 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.702 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.130 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 124206 | 17767 |
| <I/σ(I)> | 14.19 | |
| Completeness [%] | 95.8 | |
| Redundancy | 4.62 | |
| CC(1/2) | 0.999 | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 1.0 - 1.25 M ammonium sulfate and 0.1 M sodium acetate pH 4.0 - 5.5 |
