9B1R

Functional implication of the homotrimeric multidomain vacuolar sorting receptor 1 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2001-05-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0108
Spacegroup name	P 21 3
Unit cell lengths	145.543, 145.543, 145.543
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.390 - 3.500
R-factor	0.1839
Rwork	0.180
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.870
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.390	3.625
High resolution limit [Å]	3.500	3.500
Number of reflections	24159	1125
<I/σ(I)>	21.72
Completeness [%]	98.2
Redundancy	4.5
CC(1/2)	0.997	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	PEG, Tris