9B0A
GA10 nanobody bound to 2C7 peptide mimitope of Neisseria gonorrhoeae lipooligosaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953728 |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.878, 31.631, 69.783 |
| Unit cell angles | 88.86, 87.08, 89.24 |
Refinement procedure
| Resolution | 34.840 - 1.870 |
| R-factor | 0.1994 |
| Rwork | 0.198 |
| R-free | 0.22890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.039 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.840 | 1.964 |
| High resolution limit [Å] | 1.868 | 1.868 |
| Rmerge | 0.052 | 0.316 |
| Rmeas | 0.069 | 0.416 |
| Rpim | 0.046 | 0.269 |
| Number of reflections | 13142 | 657 |
| <I/σ(I)> | 8.1 | 2.1 |
| Completeness [%] | 89.0 | 77.8 |
| Redundancy | 2.1 | 2.1 |
| CC(1/2) | 0.995 | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | 0.1 M MES pH 7.0, 22 % PEG 4,000 |
