9AZZ
Crystal structure of outer membrane lipoprotein carrier protein (LolA) from Ehrlichia ruminantium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-03 |
| Detector | DECTRIS EIGER2 XE 9M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.647, 53.021, 80.802 |
| Unit cell angles | 90.00, 116.96, 90.00 |
Refinement procedure
| Resolution | 42.700 - 2.000 |
| R-factor | 0.2182 |
| Rwork | 0.216 |
| R-free | 0.26180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.076 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_5250: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.700 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.163 | 1.293 |
| Rmeas | 0.178 | 1.397 |
| Rpim | 0.069 | 0.525 |
| Total number of observations | 279537 | 21518 |
| Number of reflections | 41393 | 3080 |
| <I/σ(I)> | 6.5 | 1.5 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.8 | 7 |
| CC(1/2) | 0.990 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | JCSG+ A8: 0.2M Ammonium Formate, pH 4.6, 20% PEG 3350, EhruA.17554.a.VH2.PW39214 at 22.3 mg/mL. AC Plate 21 LolA A10. Puck: PSL-2103, Cryo: 80% crystallant + 20% glycerol. Density consistent with a PEG fragment observed in subunit B |
